CKR-5 (phospho Ser336) rabbit pAb
CKR-5 (phospho Ser336) rabbit pAb from ELK Biotechnology Read more ›
Product Description
CKR-5 (phospho Ser336) rabbit pAb is an ELK Biotechnology antibody for research workflows involving CKR-5.
Background
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemok
Additional antibody information
| Specificity | Phospho-CKR-5 (S336) Polyclonal Antibody detects endogenous levels of CKR-5 protein only when phosphorylated at S336. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using Phospho-CKR-5 (S336) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, using CCR5 (Phospho-Ser336) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | CKR-5 |
| Also known as | CCR5; CMKBR5; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CHEMR13; HIV-1 fusion coreceptor; CD antigen CD195 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 40kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Viral life cycle - HIV-1;>>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>Chemokine signaling pathway;>>Endocytosis;>>Toxoplasmosis;>>Human cytomegalovirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Human immunodeficiency virus 1 infection;>>Viral carcinogenesis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CCR5 around the phosphorylation site of Ser336. AA range:302-351 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.