ABM98343
KIF2C (phospho Ser95) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
KIF2C (phospho Ser95) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
KIF2C (phospho Ser95) rabbit pAb is an ELK Biotechnology antibody for research workflows involving KIF2C.
Background
kinesin family member 2C(KIF2C) Homo sapiens This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
Additional antibody information
| Specificity | Phospho-KIF2C (S95) Polyclonal Antibody detects endogenous levels of KIF2C protein only when phosphorylated at S95. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using KIF2C (Phospho-Ser95) Antibody | Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells treated with TNF 10ng/ml 30', using KIF2C (Phospho-Ser95) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: KIF2C
| Target | KIF2C |
| Also known as | KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse |
| Observed band | 80kD |
| Cellular localization | Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KIF2C around the phosphorylation site of Ser95. AA range:61-110 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
KIF2C
Also known as
KIF2C; KNSL6; Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human KIF2C around the phosphorylation site of Ser95. AA range:61-110
Observed band
80kD
Cellular localization
Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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