UBE3C rabbit pAb
UBE3C rabbit pAb from ELK Biotechnology Read more ›
Product Description
UBE3C rabbit pAb is an ELK Biotechnology antibody for research workflows involving UBE3C.
Background
function:E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.,miscellaneous:A cysteine residue is required for ubiquitin-thioester formation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 1 IQ domain.,subunit:Interacts with 26S proteasomes. Binds CAND2.,tissue specificity:Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.,
Additional antibody information
| Specificity | UBE3C Polyclonal Antibody detects endogenous levels of UBE3C protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using UBE3C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western Blot analysis of HuvEc cells using UBE3C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
Key Facts
| Target | UBE3C |
| Also known as | UBE3C; KIAA0010; KIAA10; Ubiquitin-protein ligase E3C; HectH2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 125kD |
| Cellular localization | proteasome complex,intracellular,nucleus,cytoplasm, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ubiquitin mediated proteolysis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from the Internal region of human UBE3C. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.