PIPK I γ rabbit pAb from ELK Biotechnology Read more ›
PIPK I γ rabbit pAb is a research-use antibody from ELK Biotechnology for studying PIPK I γ in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
phosphatidylinositol-4-phosphate 5-kinase type 1 gamma(PIP5K1C) Homo sapiens This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010],
| Specificity | PIPK I γ Polyclonal Antibody detects endogenous levels of PIPK I γ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PIPK I γ Polyclonal Antibody | Western Blot analysis of A549 cells using PIPK I γ Polyclonal Antibody | Western blot analysis of lysates from A549 cells, using PIP5K1C Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using PIP5K1C antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3212 |
| Target | PIPK I γ |
| Research areas | >>Inositol phosphate metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Endocytosis, >>Focal adhesion, >>Fc gamma R-mediated phagocytosis, >>Regulation of actin cytoskeleton, >>Yersinia infection, >>Choline metabolism in cancer |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PIP5K1C. AA range:305-354 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1: Isoform 1 is strongly expressed in brain and also detected in heart and lung. ; Isoform 2: Isoform 2 is strongly expressed in pancreas and liver and in lesser quantities in brain, heart, lung and kidney. ; Isoform 3: Isoform 3 is detected in large amounts in heart and large intestine, is also present in lung, pancreas and thyroid, and to a lesser extent in brain, stomach and kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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