PAKα rabbit pAb from ELK Biotechnology Read more ›
PAKα rabbit pAb is a research-use antibody from ELK Biotechnology for studying PAK1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010],
| Specificity | PAKα Polyclonal Antibody detects endogenous levels of PAKα protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PAKα Polyclonal Antibody diluted at 1:500 | Immunofluorescence analysis of HeLa cells, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using PAK1 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3146 |
| Target | PAK1 |
| Research areas | >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Axon guidance, >>Hippo signaling pathway - multiple species, >>Focal adhesion, >>C-type lectin receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Regulation of actin cytoskeleton, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Salmonella infection, >>Human immunodeficiency virus 1 infection, >>Proteoglycans in cancer, >>Renal cell carcinoma |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAK1. AA range:178-227 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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