ABM97316
IL-7R rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
IL-7R rabbit pAb from ELK Biotechnology Read more ›
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Product Description
IL-7R rabbit pAb is an ELK Biotechnology antibody for research workflows involving IL-7R.
Background
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015],
Additional antibody information
| Specificity | IL-7R Polyclonal Antibody detects endogenous levels of IL-7R protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using IL-7R Polyclonal Antibody | Western Blot analysis of K562 cells using IL-7R Polyclonal Antibody | Western blot analysis of lysates from K562 and COS cells, treated with insulin 0.01U/ml 15', using IL-7R/CD127 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using IL-7R/CD127 antibody. |
Key Facts
Product type
Primary Antibody›
Target: IL-7R
| Target | IL-7R |
| Also known as | IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Monkey |
| Observed band | 52 60kD |
| Cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cell membrane; Single-pass type I membrane protein.; Isoform 4: Secreted. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cytokine-cytokine receptor interaction;>>FoxO signaling pathway;>>PI3K-Akt signaling pathway;>>JAK-STAT signaling pathway;>>Hematopoietic cell lineage;>>Pathways in cancer;>>Primary immunodeficiency |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-7R/CD127. AA range:410-459 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
IL-7R
Also known as
IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-7R/CD127. AA range:410-459
Observed band
52 60kD
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cell membrane; Single-pass type I membrane protein.; Isoform 4: Secreted.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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