ABM97144
EF-1 γ rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
EF-1 γ rabbit pAb from ELK Biotechnology Read more ›
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Product Description
EF-1 γ rabbit pAb is an ELK Biotechnology antibody for research workflows involving EF-1 γ.
Background
eukaryotic translation elongation factor 1 gamma(EEF1G) Homo sapiens This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | EF-1 γ Polyclonal Antibody detects endogenous levels of EF-1 γ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using EF-1 γ Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HUVEC and HepG2 cells, using EEF1G Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using EEF1G antibody. |
Key Facts
Product type
Primary Antibody›
Target: EF-1 γ
| Target | EF-1 γ |
| Also known as | EEF1G; EF1G; Elongation factor 1-gamma; EF-1-gamma; eEF-1B gamma |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 55kD |
| Cellular localization | nucleus,cytoplasm,endoplasmic reticulum,cytosol,cell-cell adherens junction,membrane,extracellular exosome, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Legionellosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EEF1G. AA range:101-150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
EF-1 γ
Also known as
EEF1G; EF1G; Elongation factor 1-gamma; EF-1-gamma; eEF-1B gamma
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human EEF1G. AA range:101-150
Observed band
55kD
Cellular localization
nucleus,cytoplasm,endoplasmic reticulum,cytosol,cell-cell adherens junction,membrane,extracellular exosome,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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