ABM97084
SGT1/ECD Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
SGT1/ECD Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
SGT1/ECD Rabbit pAb is an ELK Biotechnology antibody for research workflows involving SGT1/ECD.
Additional antibody information
| Specificity | This antibody detects endogenous levels of SGT1/ECD at Human, Mouse,Rat |
|---|---|
| Validation evidence captions | Western blot analysis of 1)Hela Cell, 2) 293T Cell, 3)Mouse Brain Tissue, 4) Rat Brain Tissue Lysate using SGT1/ECDRabbit polyclonal Antibody diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: SGT1/ECD
| Target | SGT1/ECD |
| Also known as | Protein SGT1 (hSGT1) (Protein ecdysoneless homolog) (Suppressor of GCR2) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p1:50-200 ,WB 1:1000-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 73kD |
| Cellular localization | Cytoplasm . Nucleus . Predominantly is located in the cytoplasm. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.81% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | IHC-p1:50-200 ,WB 1:1000-2000 |
| Immunogen | Synthesized peptide derived from human SGT1/ECD AA range: 236-286 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
SGT1/ECD
Also known as
Protein SGT1 (hSGT1) (Protein ecdysoneless homolog) (Suppressor of GCR2)
Concentration
1 mg/ml
Working concentration
IHC-p1:50-200 ,WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.81% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human SGT1/ECD AA range: 236-286
Observed band
73kD
Cellular localization
Cytoplasm . Nucleus . Predominantly is located in the cytoplasm. .
Dilution range
IHC-p1:50-200 ,WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.81% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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