ABM97073
ACE2 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ACE2 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ACE2 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving ACE2.
Background
angiotensin I converting enzyme 2(ACE2) Homo sapiens The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Additional antibody information
| Specificity | This antibody detects endogenous levels of ACE2 at Human, Mouse,Rat |
|---|---|
| Validation evidence captions | Western blot analysis of 293T transfected ACE2 cell Lysate using Rabbit pAb diluted at 1:5,000. | Western blot analysis of HepG2 cell Lysate using Rabbit pAb diluted at 1:2,000 | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: ACE2
| Target | ACE2 |
| Also known as | Angiotensin-converting enzyme 2 (EC 3.4.17.23) (ACE-related carboxypeptidase) (Angiotensin-converting enzyme homolog) (ACEH) (Metalloprotease MPROT15) Cleaved into: Processed angiotensin-converting enzyme 2 |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p1:50-200 ,WB 1:1000-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 120-135kD |
| Cellular localization | Processed angiotensin-converting enzyme 2: Secreted .; Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Cell projection, cilium . Apical cell membrane . Detected in both cell membrane and cytoplasm in neurons. .; Isoform 2: Apical cell membrane . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.06% sodium azide. |
| Research area | >>Renin-angiotensin system;>>Protein digestion and absorption;>>Coronavirus disease - COVID-19 |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | IHC-p1:50-200 ,WB 1:1000-2000 |
| Immunogen | Synthesized peptide derived from human ACE2 AA range: 425-475 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
ACE2
Also known as
Angiotensin-converting enzyme 2 (EC 3.4.17.23) (ACE-related carboxypeptidase) (Angiotensin-converting enzyme homolog) (ACEH) (Metalloprotease MPROT15) Cleaved into: Processed angiotensin-converting enzyme 2
Concentration
1 mg/ml
Working concentration
IHC-p1:50-200 ,WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.06% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human ACE2 AA range: 425-475
Observed band
120-135kD
Cellular localization
Processed angiotensin-converting enzyme 2: Secreted .; Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Cell projection, cilium . Apical cell membrane . Detected in both cell membrane and cytoplasm in neurons. .; Isoform 2: Apical cell membrane .
Dilution range
IHC-p1:50-200 ,WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.06% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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