Crystallin-αC rabbit pAb
Crystallin-αC rabbit pAb from ELK Biotechnology Read more ›
Product Description
Crystallin-αC rabbit pAb is an ELK Biotechnology antibody for research workflows involving HSPB8.
Background
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Crystallin-αC protein. |
|---|---|
| Validation evidence captions | Western blot analysis of Hela using Crystallin-αC Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | HSPB8 |
| Also known as | HSPB8; CRYAC; E2IG1; HSP22; PP1629; Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22 |
| Working concentration | WB: 1:500-1000 |
| Species reactivity | Human |
| Observed band | 22kD |
| Cellular localization | Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:500-1000 |
| Immunogen | Recombinant Protein of Crystallin-αC |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.