CUL-2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CUL-2.
Background
function:Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).,pathway:Protein modification; protein ubiquitination.,PTM:CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.,similarity:Belongs to the cullin family.,subunit:Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL2, Elongin BC (TCEB1 and TCEB2), RBX1 and a variable substrate-specific adapter. Component of the ECS(VHL) or CBC(VHL) complex containing VHL. Component of the ECS(MED8) complex with the probable substrate recognition component MED8 (By similarity). Component of the ECS(PPIL5) complex with the probable substrate recognition component PPIL5.
Additional antibody information
| Specificity |
CUL-2 Polyclonal Antibody detects endogenous levels of CUL-2 protein. |
| Validation evidence captions |
Western Blot analysis of various cells using CUL-2 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of Jurkat cells using CUL-2 Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cullin 2 Antibody. The picture on the right is blocked with the synthesized peptide. |
