Histone H2A.X (Phospho Tyr142) rabbit pAb
Histone H2A.X (Phospho Tyr142) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Histone H2A.X (Phospho Tyr142) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H2A.X.
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],
Additional antibody information
| Specificity | The antibody detects endogenous Histone H2A.X (Phospho Tyr142) protein. |
|---|---|
| Validation evidence captions | Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
Key Facts
| Target | Histone H2A.X |
| Also known as | H2AFX; H2AX; Histone H2A.x; H2a/x |
| Working concentration | WB: 1:1000-2000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 15kD |
| Cellular localization | Nucleus . Chromosome . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Necroptosis;>>Neutrophil extracellular trap formation;>>Alcoholism;>>Systemic lupus erythematosus |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:1000-2000 |
| Immunogen | Synthetic Peptide of Histone H2A.X (Phospho Tyr142) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.