Histone H1 (Tri Methyl Lys25) rabbit pAb
Histone H1 (Tri Methyl Lys25) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Histone H1 (Tri Methyl Lys25) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H1.
Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015],
Additional antibody information
| Specificity | The antibody detects endogenous Histone H1 (Tri Methyl Lys25) protein. |
|---|---|
| Validation evidence captions | Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | Histone H1 |
| Also known as | H1K25ME3; HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4 |
| Working concentration | WB: 1:1000-2000 |
| Species reactivity | Human |
| Observed band | 17-25kD |
| Cellular localization | Nucleus . Chromosome . Mainly localizes in euchromatin. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:1000-2000 |
| Immunogen | Synthetic Peptide of Histone H1 (Tri Methyl Lys25) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000-2000 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.