Histone H2A.Z (Acetyl Lys7) rabbit pAb
Histone H2A.Z (Acetyl Lys7) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Histone H2A.Z (Acetyl Lys7) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H2A.Z.
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein. |
|---|---|
| Validation evidence captions | Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
Key Facts
| Target | Histone H2A.Z |
| Also known as | H2AFZ; H2AZ; Histone H2A.Z; H2A/z |
| Working concentration | WB: 1:500-1000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 14kD |
| Cellular localization | Nucleus. Chromosome. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Necroptosis;>>Neutrophil extracellular trap formation;>>Alcoholism;>>Systemic lupus erythematosus |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:500-1000 |
| Immunogen | Synthetic Peptide of Histone H2A.Z (Acetyl Lys7) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.