14-3-3σ (Phospho Ser186) rabbit pAb
14-3-3σ (Phospho Ser186) rabbit pAb from ELK Biotechnology Read more ›
Product Description
14-3-3σ (Phospho Ser186) rabbit pAb is an ELK Biotechnology antibody for research workflows involving 14-3-3σ.
Background
function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.,function:p53-regulated inhibitor of G2/M progression.,similarity:Belongs to the 14-3-3 family.,subcellular location:May be secreted by a non-classical secretory pathway.,subunit:Homodimer. Interacts with KRT17 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN.,tissue specificity:Present mainly in tissues enriched in stratified squamous keratinising epithelium.,
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human,Mouse 14-3-3σ (Phospho Ser186) |
|---|---|
| Validation evidence captions | Western Blot analysis of 1 A431 cell 2 LPS 100ng/mL 30min treated ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | 14-3-3σ |
| Also known as | 14-3-3 protein sigma (Epithelial cell marker protein 1;Stratifin) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 ELISA 1:5000-20000 |
| Species reactivity | Human;Mouse |
| Observed band | 30kD |
| Cellular localization | Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell cycle;>>p53 signaling pathway;>>Aldosterone-regulated sodium reabsorption |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:1000-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from human 14-3-3σ (Phospho Ser186) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.