CD298 rabbit pAb
CD298 rabbit pAb from ELK Biotechnology Read more ›
Product Description
CD298 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD298.
Background
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subun
Additional antibody information
| Specificity | CD298 Polyclonal Antibody detects endogenous levels of CD298 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using CD298 Polyclonal Antibody diluted at 1:1000 |
Key Facts
| Target | CD298 |
| Also known as | ATP1B3; Sodium/potassium-transporting ATPase subunit beta-3; Sodium/potassium-dependent ATPase subunit beta-3; ATPB-3; CD antigen CD298 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 31kD |
| Cellular localization | Apical cell membrane ; Single-pass type II membrane protein . Basolateral cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Cardiac muscle contraction;>>Adrenergic signaling in cardiomyocytes;>>Insulin secretion;>>Thyroid hormone synthesis;>>Thyroid hormone signaling pathway;>>Aldosterone synthesis and secretion;>>Aldosterone-regulated sodium reabsorption;>>Endocrine and other factor-regulated calcium reabsorption;>>Proximal tubule bicarbonate reclamation;>>Salivary secretion;>>Gastric acid secretion;>>Pancreatic secretion;>>Carbohydrate digestion and absorption;>>Protein digestion and absorption;>>Bile secretion;>>Mineral absorption |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from Human N-ternal CD298 . at AA range: 60-140 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.