Primary Antibody ISO 9001|ABMIUM Verified™
ABM96346

COLGALT1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

COLGALT1 rabbit pAb from ELK Biotechnology Read more ›

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COLGALT1 rabbit pAb
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COLGALT1 rabbit pAb

Product Description

COLGALT1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving COLGALT1.

Background

The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum. [provided by RefSeq, Dec 2015],

Additional antibody information

Specificity This antibody detects endogenous levels of COLGALT1 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product type Primary Antibody Target: COLGALT1
Target COLGALT1
Also known as Procollagen galactosyltransferase 1 (EC 2.4.1.50) (Glycosyltransferase 25 family member 1) (Hydroxylysine galactosyltransferase 1)
Concentration 1 mg/ml
Working concentration WB 1:500-2000
Species reactivity Human; Mouse
Observed band 75kD
Cellular localization Endoplasmic reticulum lumen . Colocalized with PLOD3 and mannose binding lectin/MBL2. .
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.
Research area >>Lysine degradation;>>Other types of O-glycan biosynthesis;>>Metabolic pathways
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WB
Dilution range WB 1:500-2000
Immunogen Synthesized peptide derived from human COLGALT1 AA range: 194-244
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
COLGALT1
Also known as
Procollagen galactosyltransferase 1 (EC 2.4.1.50) (Glycosyltransferase 25 family member 1) (Hydroxylysine galactosyltransferase 1)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human COLGALT1 AA range: 194-244
Observed band
75kD
Cellular localization
Endoplasmic reticulum lumen . Colocalized with PLOD3 and mannose binding lectin/MBL2. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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