ABM96346
COLGALT1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
COLGALT1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
COLGALT1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving COLGALT1.
Background
The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum. [provided by RefSeq, Dec 2015],
Additional antibody information
| Specificity | This antibody detects endogenous levels of COLGALT1 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: COLGALT1
| Target | COLGALT1 |
| Also known as | Procollagen galactosyltransferase 1 (EC 2.4.1.50) (Glycosyltransferase 25 family member 1) (Hydroxylysine galactosyltransferase 1) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse |
| Observed band | 75kD |
| Cellular localization | Endoplasmic reticulum lumen . Colocalized with PLOD3 and mannose binding lectin/MBL2. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide. |
| Research area | >>Lysine degradation;>>Other types of O-glycan biosynthesis;>>Metabolic pathways |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human COLGALT1 AA range: 194-244 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
Specifications
Target
COLGALT1
Also known as
Procollagen galactosyltransferase 1 (EC 2.4.1.50) (Glycosyltransferase 25 family member 1) (Hydroxylysine galactosyltransferase 1)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human COLGALT1 AA range: 194-244
Observed band
75kD
Cellular localization
Endoplasmic reticulum lumen . Colocalized with PLOD3 and mannose binding lectin/MBL2. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.04% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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