Doublecortin (phospho-Ser297) rabbit pAb
Doublecortin (phospho-Ser297) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Doublecortin (phospho-Ser297) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Doublecortin.
Background
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain&quo
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Rat Doublecortin (phospho-Ser297) |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | Doublecortin |
| Also known as | Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Rat |
| Observed band | 44kD |
| Cellular localization | Cytoplasm . Cell projection, neuron projection . Localizes at neurite tips. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human Doublecortin (Ser297) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.