EPHA1 (Phospho-Tyr605) rabbit pAb
EPHA1 (Phospho-Tyr605) rabbit pAb from ELK Biotechnology Read more ›
Product Description
EPHA1 (Phospho-Tyr605) rabbit pAb is an ELK Biotechnology antibody for research workflows involving EPHA1.
Background
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous phospho levels of EPHA1 (Phospho-Tyr605) at Human:Y605, Mouse:Y606 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of 1 Hela treated with LPS, 2 Hela,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | EPHA1 |
| Also known as | Ephrin type-A receptor 1 (hEpha1;EC 2.7.10.1;EPH tyrosine kinase;EPH tyrosine kinase 1;Erythropoietin-producing hepatoma receptor;Tyrosine-protein kinase receptor EPH) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, WB 1:500-2000 |
| Species reactivity | Human; Mouse |
| Observed band | 108kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Axon guidance |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | IHC-p 1:50-200, WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human EPHA1 (Phospho-Tyr605) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.