F111A rabbit pAb
F111A rabbit pAb from ELK Biotechnology Read more ›
Product Description
F111A rabbit pAb is an ELK Biotechnology antibody for research workflows involving F111A.
Background
The protein encoded by this gene is cell-cycle regulated, and has nuclear localization. The C-terminal half of the protein shares homology with trypsin-like peptidases and it contains a PCNA-interacting peptide (PIP) box, that is necessary for its co-localization with proliferating cell nuclear antigen (PCNA). Reduced expression of this gene resulted in DNA replication defects, consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Mutations in this gene have been associated with Kenny-Caffey syndrome (KCS) type 2 and the more severe osteocraniostenosis (OCS, also known as Gracile Bone Dysplasia), both characterized by short stature, hypoparathyroidism, bone development abnormalities, and hypocalcemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],
Additional antibody information
| Specificity | This antibody detects endogenous levels of F111A at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | F111A |
| Concentration | 1 mg/ml |
| Working concentration | WB 1：500-2000 |
| Species reactivity | Human; Mouse |
| Cellular localization | Nucleus . Chromosome . Cytoplasm . Mainly localizes to nucleus: colocalizes with PCNA on replication sites. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1：500-2000 |
| Immunogen | Synthesized peptide derived from human F111A AA range: 264-314 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.