ABHD7 rabbit pAb
ABHD7 rabbit pAb from ELK Biotechnology Read more ›
Product Description
ABHD7 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ABHD7.
Background
The α/β hydrolase superfamily comprise diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical α/β hydrolase fold, which consists of an eight-stranded parallel α/β structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer's disease, obesity and blood clotting disorders. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members, most of which utilize a serine nucleophile to form the G-X-S-X-G nucleophile elbow. ABHD7 is a 362 amino acid single-pass type II membrane protein that belongs to the AB hydrolase superfamily and the Epoxide hydrolase family.
Additional antibody information
| Specificity | ABHD7 Polyclonal Antibody detects endogenous levels of ABHD7 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using ABHD7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 cells, using ABHD7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using ABHD7 antibody. |
Key Facts
| Target | ABHD7 |
| Also known as | EPHX4; ABHD7; EPHXRP; Epoxide hydrolase 4; Abhydrolase domain-containing protein 7; Epoxide hydrolase-related protein |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 42kD |
| Cellular localization | Membrane ; Single-pass type II membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Chemical carcinogenesis - receptor activation;>>Chemical carcinogenesis - reactive oxygen species |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ABHD7. AA range:101-150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.