Histone H2B (phospho-Ser14) rabbit pAb
Histone H2B (phospho-Ser14) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Histone H2B (phospho-Ser14) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H2B.
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Histone H2B (phospho-Ser14) |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | Histone H2B |
| Also known as | Histone H2B type 1-H (Histone H2B.j) (H2B/j) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 18kD |
| Cellular localization | Nucleus. Chromosome. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis;>>Systemic lupus erythematosus |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human Histone H2B (Ser14) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.