ABCA8 rabbit pAb
ABCA8 rabbit pAb from ELK Biotechnology Read more ›
Product Description
ABCA8 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ABCA8.
Background
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The encoded protein may regulate lipid metabolism and be involved in the formation and maintenance of myelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
Additional antibody information
| Specificity | ABCA8 Polyclonal Antibody detects endogenous levels of ABCA8 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using ABCA8 Polyclonal Antibody | Western Blot analysis of HuvEc cells using ABCA8 Polyclonal Antibody | Western blot analysis of lysates from HUVEC cells, using ABCA8 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | ABCA8 |
| Also known as | ABCA8; KIAA0822; ATP-binding cassette sub-family A member 8 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 179kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane . Predominantly expressed on the sinusoidal plasma membrane. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>ABC transporters |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ABCA8. AA range:1151-1200 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.