ABM95601
17β-HSD11 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
17β-HSD11 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
17β-HSD11 rabbit pAb is an ELK Biotechnology antibody for research workflows involving 17β-HSD11.
Background
Short-chain alcohol dehydrogenases, such as HSD17B11, metabolize secondary alcohols and ketones (Brereton et al., 2001 [PubMed 11165019]).[supplied by OMIM, Jun 2009],
Additional antibody information
| Specificity | 17β-HSD11 Polyclonal Antibody detects endogenous levels of 17β-HSD11 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of various cells using 17β-HSD11 Polyclonal Antibody | Western blot analysis of lysates from 293 cells, using DHRS8 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from RAW264.7cells using DHRS8 antibody. |
Key Facts
Product type
Primary Antibody›
Target: 17β-HSD11
| Target | 17β-HSD11 |
| Also known as | HSD17B11; DHRS8; PAN1B; PSEC0029; Estradiol 17-beta-dehydrogenase 11; 17-beta-hydroxysteroid dehydrogenase 11; 17-beta-HSD 11; 17bHSD11; 17betaHSD11; 17-beta-hydroxysteroid dehydrogenase XI; 17-beta-HSD XI; 17betaHSDXI; Cutaneous T-cell lym |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 36kD |
| Cellular localization | Endoplasmic reticulum . Lipid droplet . Redistributed from the endoplasmic reticulum to lipids droplets in the cell upon induction of lipids droplet formation. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DHRS8. AA range:71-120 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
17β-HSD11
Also known as
HSD17B11; DHRS8; PAN1B; PSEC0029; Estradiol 17-beta-dehydrogenase 11; 17-beta-hydroxysteroid dehydrogenase 11; 17-beta-HSD 11; 17bHSD11; 17betaHSD11; 17-beta-hydroxysteroid dehydrogenase XI; 17-beta-HSD XI; 17betaHSDXI; Cutaneous T-cell lym
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human DHRS8. AA range:71-120
Observed band
36kD
Cellular localization
Endoplasmic reticulum . Lipid droplet . Redistributed from the endoplasmic reticulum to lipids droplets in the cell upon induction of lipids droplet formation. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. IF 1:100-300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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