p38 (phospho Thr180/Y182) rabbit pAb
p38 (phospho Thr180/Y182) rabbit pAb from ELK Biotechnology Read more ›
Product Description
p38 (phospho Thr180/Y182) rabbit pAb is an ELK Biotechnology antibody for research workflows involving p38.
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d
Additional antibody information
| Specificity | Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182. |
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Key Facts
| Target | p38 |
| Also known as | MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein |
| Concentration | 1 mg/ml |
| Working concentration | IF/ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;ELISA 1:5000-20000 |
| Species reactivity | Human;Mouse;Rat;Guineapig |
| Observed band | 38kD |
| Cellular localization | Cytoplasm . Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocrine resistance;>>MAPK signaling pathway;>>Rap1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Neurotrophin signaling pathway;>>Retrograde endocannabinoid signaling;>>Dopaminergic synapse;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Progesterone-mediated oocyte maturation;> |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | IF/ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;ELISA 1:5000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Guineapig |
| Species | Dilution | Notes |
|---|---|---|
| Human | IF/ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Guineapig | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.