ABM94972
Ephrin-B1/2 (phospho Tyr330) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Ephrin-B1/2 (phospho Tyr330) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Ephrin-B1/2 (phospho Tyr330) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Ephrin-B1/2.
Background
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-Ephrin-B1/2 (Y330) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2 protein only when phosphorylated at Y330. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Ephrin-B1/2 (Y330) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using EFNB1/2 (Phospho-Tyr330) Antibody | Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using EFNB1/2 (Phospho-Tyr330) Antibody. The lane on the left is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: Ephrin-B1/2
| Target | Ephrin-B1/2 |
| Also known as | EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 59kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; Ephrin-B1 C-terminal fragment: Cell membrane ; Single-pass type I membrane protein .; Ephrin-B1 intracellular domain: Nucleus . Colocalizes with ZHX2 in the nucleus. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Axon guidance |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyr330. AA range:284-333 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Ephrin-B1/2
Also known as
EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyr330. AA range:284-333
Observed band
59kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; Ephrin-B1 C-terminal fragment: Cell membrane ; Single-pass type I membrane protein .; Ephrin-B1 intracellular domain: Nucleus . Colocalizes with ZHX2 in the nucleus. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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