Primary Antibody ISO 9001|ABMIUM Verified™
ABM94844

Prdx1 (phospho-Tyr194) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Prdx1 (phospho-Tyr194) rabbit pAb from ELK Biotechnology Read more ›

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Prdx1 (phospho-Tyr194) rabbit pAb
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Prdx1 (phospho-Tyr194) rabbit pAb

Product Description

Prdx1 (phospho-Tyr194) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Prdx1.

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Prdx1 (phospho-Tyr194)
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: Prdx1
Target Prdx1
Also known as Peroxiredoxin-1 (EC 1.11.1.15) (Natural killer cell-enhancing factor A) (NKEF-A) (Proliferation-associated gene protein) (PAG) (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2)
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Species reactivity Human;Rat;Mouse;
Observed band 21kD
Cellular localization Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>Peroxisome;>>Amoebiasis
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCELISA
Dilution range WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen Synthesized phosho peptide around human Prdx1 (Tyr194)
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Prdx1
Also known as
Peroxiredoxin-1 (EC 1.11.1.15) (Natural killer cell-enhancing factor A) (NKEF-A) (Proliferation-associated gene protein) (PAG) (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCELISA
Immunogen
Synthesized phosho peptide around human Prdx1 (Tyr194)
Observed band
21kD
Cellular localization
Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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