Primary Antibody ISO 9001|ABMIUM Verified™
ABM94706

RHBT2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

RHBT2 rabbit pAb from ELK Biotechnology Read more ›

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RHBT2 rabbit pAb
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RHBT2 rabbit pAb

Product Description

RHBT2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving RHBT2.

Background

The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011],

Additional antibody information

Specificity This antibody detects endogenous levels of RHBT2 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: RHBT2
Target RHBT2
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Species reactivity Human; Mouse
Cellular localization intracellular,cytosol,plasma membrane,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>Ubiquitin mediated proteolysis
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCELISA
Dilution range WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen Synthesized peptide derived from human RHBT2 AA range: 573-623
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
RHBT2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WBIHCELISA
Immunogen
Synthesized peptide derived from human RHBT2 AA range: 573-623
Cellular localization
intracellular,cytosol,plasma membrane,
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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