ABM94652
ICAM-1 (phospho Tyr512) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ICAM-1 (phospho Tyr512) rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
ICAM-1 (phospho Tyr512) rabbit pAb is an ELK Biotechnology antibody for research workflows involving ICAM-1.
Background
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-ICAM-1 (Y512) Polyclonal Antibody detects endogenous levels of ICAM-1 protein only when phosphorylated at Y512. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-ICAM-1 (Y512) Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HeLa cells treated with TNF-alpha 20ng/ml 5', using ICAM-1 (Phospho-Tyr512) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: ICAM-1
| Target | ICAM-1 |
| Also known as | ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 57kD |
| Cellular localization | Membrane; Single-pass type I membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>NF-kappa B signaling pathway;>>Cell adhesion molecules;>>Natural killer cell mediated cytotoxicity;>>TNF signaling pathway;>>Leukocyte transendothelial migration;>>AGE-RAGE signaling pathway in diabetic complications;>>African trypanosomiasis;>>Malaria;>>Staphylococcus aureus infection;>>Influenza A;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Rheumatoid arthritis;>>Viral myocarditis;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyr512. AA range:479-528 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
ICAM-1
Also known as
ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyr512. AA range:479-528
Observed band
57kD
Cellular localization
Membrane; Single-pass type I membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed