Caveolin-1 (phospho Tyr14) rabbit pAb
Caveolin-1 (phospho Tyr14) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Caveolin-1 (phospho Tyr14) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Caveolin-1.
Background
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010],
Additional antibody information
| Specificity | Phospho-Caveolin-1 (Y14) Polyclonal Antibody detects endogenous levels of Caveolin-1 protein only when phosphorylated at Y14. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HeLa cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30' and HeLa cells treated with LPS 100ng/ml 30', using Caveolin-1 (Phospho-Tyr14) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Caveolin-1 |
| Also known as | CAV1; CAV; Caveolin-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 20kD |
| Cellular localization | Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola ; Peripheral membrane protein. Membrane raft . Golgi apparatus, trans-Golgi network . Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocytosis;>>Focal adhesion;>>Prion disease;>>Bacterial invasion of epithelial cells;>>Proteoglycans in cancer;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caveolin-1 around the phosphorylation site of Tyr14. AA range:5-54 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.