ABM92877
Cleaved-MMP-27 (Y99) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Cleaved-MMP-27 (Y99) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Cleaved-MMP-27 (Y99) rabbit pAb is an ELK Biotechnology antibody for research workflows involving MMP-27.
Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Cleaved-MMP-27 (Y99) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-27 protein resulting from cleavage adjacent to Y99. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-MMP-27 (Y99) Polyclonal Antibody | Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using MMP27 (Cleaved-Tyr99) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: MMP-27
| Target | MMP-27 |
| Also known as | MMP27; Matrix metalloproteinase-27; MMP-27 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Monkey |
| Observed band | 48kD |
| Cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein . Retained in the endoplasmic reticulum. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP27. AA range:80-129 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Monkey | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
MMP-27
Also known as
MMP27; Matrix metalloproteinase-27; MMP-27
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP27. AA range:80-129
Observed band
48kD
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein . Retained in the endoplasmic reticulum. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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