Claudin-2 (8H11) Mouse mAb
Claudin-2 (8H11) Mouse mAb from ELK Biotechnology Read more ›
Product Description
Claudin-2 (8H11) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Claudin-2 (8H11).
Background
The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer . Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney, where it regulates transepithelial ion (e.g., Na+, Cl-) reabsorption . Increased expression of Claudin-2 has been reported in some cancer cell lines , including A549 lung adenocarcinoma cells, where its nuclear distribution was positively associated with enhanced proliferation .
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Heart Tissue Lysate , 3) Rat Heart Tissue Lysate using Claudin-2 （EM1403）Mouse mAb diluted at 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma Tissue using Claudin-2 （EM1403）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Endometrial Carcinoma Tissue using Claudin-2 （EM1403）Mouse mAb diluted at 1:200. |
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Key Facts
| Target | Claudin-2 (8H11) |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC:1;100-1:200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 25kDa |
| Cellular localization | Cell junction,tight junction,Cell membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC:1;100-1:200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.