Acetyl NF kB P65（K314/K315) (5G11) Mouse mAb
Acetyl NF kB P65（K314/K315) (5G11) Mouse mAb from ELK Biotechnology Read more ›
Product Description
Acetyl NF kB P65（K314/K315) (5G11) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Acetyl NF kB P65（K314/K315) (5G11).
Background
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
Additional antibody information
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl NF kB P65(K314/K315) （EM1377）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Acetyl NF kB P65(K314/K315) （EM1377）Mouse mAb diluted at 1:200. |
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Key Facts
| Target | Acetyl NF kB P65（K314/K315) (5G11) |
| Also known as | NF kappa B p65, NFKB3, p65, p65 NF kB, RELA, TF65, Transcription Factor p65 |
| Concentration | 1mg/mL |
| Working concentration | IHC 1:100-200 |
| Species reactivity | Human, Mouse, Rat |
| Observed band | 65kDa |
| Cellular localization | Cytoplasm, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
IHC |
| Dilution range | IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC 1:100-200 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.