ABM92582
ERK1/2 (1H4) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
ERK1/2 (1H4) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
ERK1/2 (1H4) Mouse mAb is an ELK Biotechnology antibody for research workflows involving ERK1/2 (1H4).
Background
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela Cell Lysate, 2)Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using ERK1/2（EM1326）Mouse mAb diluted at 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using ERK1/2（EM1326）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ERK1/2（EM1326）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: ERK1/2 (1H4)
| Target | ERK1/2 (1H4) |
| Also known as | MAPK, Mitogen-activated protein Kinase, PRKM2,MK01 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 44,42kDa |
| Cellular localization | Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
ERK1/2 (1H4)
Also known as
MAPK, Mitogen-activated protein Kinase, PRKM2,MK01
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
44,42kDa
Cellular localization
Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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