ABM92481
α skeletal muscle actin Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
α skeletal muscle actin Mouse mAb from ELK Biotechnology Read more ›
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Product Description
α skeletal muscle actin Mouse mAb is an ELK Biotechnology antibody for research workflows involving α skeletal muscle actin.
Background
Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with EM1149 diluted at 1:20,000. | 1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1149 1:10,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muscle Tissue using α skeletal muscle actin （EM1149）Mouse mAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: α skeletal muscle actin
| Target | α skeletal muscle actin |
| Also known as | ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000-10,000 IP 1:200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 42kDa |
| Cellular localization | Cytoplasm, Cytoskeleton |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:5,000-10,000 IP 1:200 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
α skeletal muscle actin
Also known as
ACTA antibody, ACTA1 antibody, ACTC antibody, ACTC1 antibody, Actin alpha cardiac muscle antibody, ACTS antibody, Alpha actin 1 antibody, Cardiac muscle alpha actin 1 antibody, Skeletal muscle alpha actin 1 antibody
Concentration
1mg/mL
Working concentration
WB 1:5,000-10,000 IP 1:200 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
42kDa
Cellular localization
Cytoplasm, Cytoskeleton
Dilution range
WB 1:5,000-10,000 IP 1:200 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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