Cytokeratin 18 Mouse mAb
Cytokeratin 18 Mouse mAb from ELK Biotechnology Read more ›
Product Description
Cytokeratin 18 Mouse mAb is an ELK Biotechnology antibody for research workflows involving Cytokeratin 18.
Background
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue with Cytokeratin 18 Mouse MAb (1B3) diluted at 1:2000. | IF analysis of Hela with EM1095(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Cytokeratin 18 （EM1095）Mouse mAb diluted at 1:500. |
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Key Facts
| Target | Cytokeratin 18 |
| Also known as | Cell proliferation inducing gene 46 protein, CK 18, CYK 18, K 18, Keratin 18, Ketatin D, KRT18 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 46kDa |
| Cellular localization | Cytoplasm, Intermediate filament, Keratin, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.