β-Tubulin Mouse mAb
β-Tubulin Mouse mAb from ELK Biotechnology Read more ›
Product Description
β-Tubulin Mouse mAb is an ELK Biotechnology antibody for research workflows involving β-Tubulin.
Background
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Additional antibody information
| Validation evidence captions | Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000. | IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200. | IF analysis of Hela with EM1029(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
| Target | β-Tubulin |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IHC 1:200 IF 1:100-200 |
| Species reactivity | Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast |
| Observed band | 55kDa |
| Cellular localization | Cytoplasm,Cytoskeleton,Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:5,000 IHC 1:200 IF 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
11 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse | |||||||
| Monkey | |||||||
| Dog | |||||||
| Chicken | |||||||
| Hamster | |||||||
| Rabbit | |||||||
| Sheep | |||||||
| Insect | |||||||
| Yeast |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Rat | — | — |
| Mouse | — | — |
| Monkey | — | — |
| Dog | — | — |
| Chicken | — | — |
| Hamster | — | — |
| Rabbit | — | — |
| Sheep | — | — |
| Insect | — | — |
| Yeast | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.