VSV-G-Tag Mouse mAb
VSV-G-Tag Mouse mAb from ELK Biotechnology Read more ›
Product Description
VSV-G-Tag Mouse mAb is an ELK Biotechnology antibody for research workflows involving VSV-G-Tag.
Background
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.
Additional antibody information
| Validation evidence captions | IP antibody use：5ug VSV-G Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Pr | IF analysis of 293T cells transfected with a VSV-G-tagged protein, using ELKbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G) | 1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 |
|---|
Key Facts
| Target | VSV-G-Tag |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Species reactivity | Epitope tag |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 target type| Target type | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Target type | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.