3-NT(3-Nitrotyrosine) ELISA Kit
3-NT(3-Nitrotyrosine) ELISA Kit from ELK Biotechnology Read more ›
Product Description
3-NT(3-Nitrotyrosine) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 3-NT.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine.
Key Facts
| Target | 3-NT |
| Also known as | 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine |
| Species reactivity | General |
| Form / buffer | Pre-coated microplate kit |
| Research area | Metabolic pathway, Infection immunity, Rheumatology |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 1.4 ng/mL |
| Detection range | 4.69-300 ng/mL |
| Sample types |
SerumPlasmaOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.