Human IL18RAP(Interleukin 18 Receptor Accessory Protein) ELISA Kit

Human IL18RAP(Interleukin 18 Receptor Accessory Protein) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of IL18RAP.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL18RAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL18RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL18RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL18RAP in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include IL-1R7;CDw218b; CD218-B;ACPL; Accessory protein-likeL; CD218 antigen-like family member B; IL-1R accessory protein-like; Interleukin-1 receptor 7; Interleukin-18 receptor beta.