Mouse VCL(Vinculin) ELISA Kit
Mouse VCL(Vinculin) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Mouse VCL(Vinculin) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of VCL.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VCL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VCL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VCL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VCL in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include MVCL; Metavinculin.
Key Facts
| Target | VCL |
| Also known as | MVCL; Metavinculin |
| Species reactivity | Mouse |
| Form / buffer | Pre-coated microplate kit |
| Research area | Metabolic pathway, Developmental science, Bone metabolism |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.132 ng/mL |
| Detection range | 0.32-20 ng/mL |
| Sample types |
Tissue homogenatesCell lysatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.