Human C8g(Complement Component 8g) ELISA Kit

Human C8g(Complement Component 8g) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of C8g.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C8g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C8g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C8g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C8g in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include C8C; C8G; CO8G_HUMAN; Complement component C8 gamma chain; MGC142186.