Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of Alk-Smase.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase.
Key Facts
| Target | Alk-Smase |
| Also known as | ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Enzyme & Kinase, Neuro science |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.046 ng/mL |
| Detection range | 0.16-10 ng/mL |
| Sample types |
Tissue homogenatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.