Rat CASP14(Caspase 14) ELISA Kit
Rat CASP14(Caspase 14) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat CASP14(Caspase 14) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of CASP14.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP14 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14.
Key Facts
| Target | CASP14 |
| Also known as | Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14 |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Apoptosis |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.114 ng/mL |
| Detection range | 0.32-20 ng/mL |
| Sample types |
Tissue homogenatesCell lysatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.