Rat COL4(Collagen Type IV) ELISA Kit
Rat COL4(Collagen Type IV) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat COL4(Collagen Type IV) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of COL4.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include Type-IV Collagen.
Key Facts
| Target | COL4 |
| Also known as | Type-IV Collagen |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Tumor immunity, Hepatology |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.259 ng/mL |
| Detection range | 0.63-40 ng/mL |
| Sample types |
SerumPlasmaTissue homogenatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.