Human IgG3(Immunoglobulin G3) ELISA Kit

Human IgG3(Immunoglobulin G3) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of IgG3.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include IGHG3; HDC; Heavy chain disease protein; Ig Gamma-3 Chain C Region; Immunoglobulin Heavy Constant Gamma 3; G3m Marker; Immunoglobulin Gm3.