Zebrafish ANP(Atrial Natriuretic Peptide) ELISA Kit
Zebrafish ANP(Atrial Natriuretic Peptide) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Zebrafish ANP(Atrial Natriuretic Peptide) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of ANP.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish ANP in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin.
Key Facts
| Target | ANP |
| Also known as | ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin |
| Species reactivity | Zebrafish |
| Form / buffer | Pre-coated microplate kit |
| Research area | Endocrinology, Cardiovascular biology, Hormone metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 4.60 pg/mL |
| Detection range | 15.63-1000 pg/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.