Pig sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit

Pig sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of sTREM-1.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1.