Human 3-MH(3-Methylhistidine) ELISA Kit
Human 3-MH(3-Methylhistidine) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Human 3-MH(3-Methylhistidine) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 3-MH.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 3-MH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 3-MH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 3-MH in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include 3MH.
Key Facts
| Target | 3-MH |
| Also known as | 3MH |
| Species reactivity | Human |
| Form / buffer | Pre-coated microplate kit |
| Research area | Metabolism |
| Assay type | Competitive Inhibition |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 2.61 nmol/mL |
| Detection range | 6.25-400 nmol/mL |
| Sample types |
SerumPlasmaTissue homogenatesOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.