Mouse E-Cad(E-Cadherin) ELISA Kit
Mouse E-Cad(E-Cadherin) ELISA Kit from ELK Biotechnology Read more ›
Product Description
Mouse E-Cad(E-Cadherin) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of E-Cad.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin.
Key Facts
| Target | E-Cad |
| Also known as | CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin |
| Species reactivity | Mouse |
| Form / buffer | Pre-coated microplate kit |
| Research area | Signal Transduction, Cancer, Developmental Biology |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 0.132 ng/mL |
| Detection range | 0.32-20 ng/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.